@article{Dalghi_Farzad Khajavi_Ahmadi_2023, title={The effect of aspirin on caspase-8 and -9 activity level in cervical cancer cells in vitro}, volume={6}, url={https://onlinejbs.com/index.php/jbs/article/view/7761}, DOI={10.62400/jbs.v6i1.7761}, abstractNote={<p>Caspases are key mediators of apoptosis. Caspase-8 mediates extrinsic, and caspase-9 initiates the intrinsic pathway of apoptosis. The literature shows that there is a relation between non-steroidal anti-inflammatory drugs (NSAIDs) and apoptosis in cancer cells. Accordingly, the current research investigated the effects of aspirin on caspase-8 and -9 activity level in cervical cancer (Hela) cells. In this in vitro study, Hela cells were divided to control untreated group and the groups treated with 0.0001, 0.001, 0.01, 1.0, 1 and 10 mg/ml of aspirin. MTT method was used to determine the cell viability. The caspase colorimetric assay method was used to detect the caspase activity level. The data were analyzed using ANOVA and Student’s t-test. The results showed that the cytotoxic concentration of aspirin significantly increased the activity level of caspase-9 (p<0.001), however, did not significantly change the caspase-8 activity level. It can be concluded that aspirin is able to initiate the intrinsic pathway of apoptosis in cervical cancer cells.</p>}, number={1}, journal={Journal of Biological Studies}, author={Dalghi, Erfaneh and Farzad Khajavi, Amir and Ahmadi, Rahim}, year={2023}, month={May}, pages={77–86} }