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The aim of the present study is to investigate the effects of single Eugenol dose on chick embryo development. Fertilized treated eggs of Gallus gallus domesticus injected with a single volume of 0.1, 0.2, 0.3, 0.4 and 0.5 μl Eugenol to the egg air chamber and control egg group were incubated at 37ºC in the humidified incubator for 3, 6, 12 and 18 days. A visible damage effect caused by increasing of Eugenol doses on the vascular development with the cause of angiogenesis and brain acetychlorinesterase (AchE) activity suppression, incomplete embryonic development with the increasing of malformation and the mortality rate of the chick embryo. This work demonstrated that the treatment of the chick embryo in the early stages with Eugenol may be considered as teratogenic agents at the dose levels utilized in our laboratory conditions between days 3 to day 18 of incubation.