The cytotoxic effects of a bioactive nitro derivative of pyrimidine to human breast cancer (MCF-7) cells

Abstract

Breast cancer is one of the leading causes of mortality among women worldwide mostly due to resistance to existing chemotherapeutic agents. In this study, a bioactive nitro derivative of pyrimidine compound 2,6-Diamino-5-((5-methylisoxazole-3-yl) diazenyl) pyrimidin-4-0l was synthesized by the condensation reaction under reflux conditions. Characteristics and structure of synthesized compound was confirmed by the FT-IR,C-NMR and H-NMR analyzes. The cytotoxic effect of the compound was measured by MTT assay at various concentrations (5-100 μg/ml) on breast cancer MCF-7 cells. The MTT results indicated that the synthesized compound had a significant cytotoxic effects on MCF-7 cells in a dose-dependent manner. Our findings indicated that bioactive nitro derivative of pyrimidine compound has a potential to be applied as anticancer agent against breast cancer cells.