The effects of berberine hydrochloride on the bone quality in methamphetamine-addicted rats after 3 weeks of withdrawal

Main Article Content

Fariba Yahyaei
Samira Behrad
Raheb Ghorbani
Mehdi Khaksari
Elham Sadat Afraz

Abstract

Animal studies have shown that berberine hydrochloride reduces osteoporosis and has anti-inflammatory effects. The aim of this study was to evaluate the therapeutic effect of berberine hydrochloride on the quality of new bone and the severity of bone inflammation in methamphetamine-addicted rats after 3 weeks of withdrawal. A total of 21 male Wistar rats were divided into 2 groups: control (n = 7), methamphetamine (n = 7), and methamphetamine + berberine groups (n = 7). The 2 addicted groups received water-soluble methamphetamine up to 12 mg/kg for 2 weeks. Subsequently, they were abstinent for 3 weeks. Only 1 group received 100 mg/ kg/ day of berberine. After 3 weeks, the mandibular bone of rats was removed for histopathological evaluation of the severity of inflammation and the quality of new bone via hematoxylin and eosin (H&E) staining. Data were analyzed using Shapiro-Wilk and Mann-Whitney U tests. The software used was SPSS version 24, and the significance level was 0.05. The new bone formation was more mature in the methamphetamine + berberine group than in the methamphetamine group (P < 0.05). The bone inflammation was more severe in the methamphetamine group than in the methamphetamine + berberine group (P < 0.05). Because of the beneficial effects of berberine on the mandibular jaw, it can reduce the oral side effects of methamphetamine addiction.

Article Details

How to Cite
Yahyaei, F., Behrad, S., Ghorbani, R., Khaksari, M., & Sadat Afraz, E. (2022). The effects of berberine hydrochloride on the bone quality in methamphetamine-addicted rats after 3 weeks of withdrawal. Journal of Biological Studies, 5(3 (Special Issue), 585–593. Retrieved from https://onlinejbs.com/index.php/jbs/article/view/7077
Section
Articles